Lineage-tracing of bronchioalveolar stem cells (BASCs) in different pulmonary disease models

Max-Planck-Institut für Herz- und Lungenforschung - W. G. Kerckhoff-Institut

Lineage-tracing of bronchioalveolar stem cells (BASCs) in different pulmonary disease models

BASCs contribute substantially to distal lung regeneration and display a bi-potent differentiation potential. Top: β‐galactosidase staining of cleared whole lung preparations isolated from control and injured BASC-specific animals (SPC-2A YFP-2A tTA N, CCSP-2A mCherry-2A tTA C, tetObiluc/Cre, Rosa26stopflox-lacZ) 21 days (bleomycin, naphthalene) or 35 days (influenza) following injury. Bottom: Sequential imaging of epifluorescence and β‐galactosidase activity, illustrating the alveolar and bronchiolar fate of BASC-derived cells. — BASCs contribute substantially to distal lung regeneration and display a bi-potent differentiation potential. Top: β‐galactosidase staining of cleared whole lung preparations isolated from control and injured BASC-specific animals (SPC^{-2A YFP-2A tTA N}, CCSP^{-2A mCherry-2A tTA C}, tetO_bi^luc/Cre, Rosa26^{stopflox-lacZ}) 21 days (bleomycin, naphthalene) or 35 days (influenza) following injury. Bottom: Sequential imaging of epifluorescence and β‐galactosidase activity, illustrating the alveolar and bronchiolar fate of BASC-derived cells.

BASCs contribute substantially to distal lung regeneration and display a bi-potent differentiation potential. Top: β‐galactosidase staining of cleared whole lung preparations isolated from control and injured BASC-specific animals (SPC^{-2A YFP-2A tTA N}, CCSP^{-2A mCherry-2A tTA C}, tetO_bi^luc/Cre, Rosa26^{stopflox-lacZ}) 21 days (bleomycin, naphthalene) or 35 days (influenza) following injury. Bottom: Sequential imaging of epifluorescence and β‐galactosidase activity, illustrating the alveolar and bronchiolar fate of BASC-derived cells.

BASCs contribute substantially to distal lung regeneration and display a bi-potent differentiation potential. Top: β‐galactosidase staining of cleared whole lung preparations isolated from control and injured BASC-specific animals (SPC-2A YFP-2A tTA N, CCSP-2A mCherry-2A tTA C, tetObiluc/Cre, Rosa26stopflox-lacZ) 21 days (bleomycin, naphthalene) or 35 days (influenza) following injury. Bottom: Sequential imaging of epifluorescence and β‐galactosidase activity, illustrating the alveolar and bronchiolar fate of BASC-derived cells. — BASCs contribute substantially to distal lung regeneration and display a bi-potent differentiation potential. Top: β‐galactosidase staining of cleared whole lung preparations isolated from control and injured BASC-specific animals (SPC^{-2A YFP-2A tTA N}, CCSP^{-2A mCherry-2A tTA C}, tetO_bi^luc/Cre, Rosa26^{stopflox-lacZ}) 21 days (bleomycin, naphthalene) or 35 days (influenza) following injury. Bottom: Sequential imaging of epifluorescence and β‐galactosidase activity, illustrating the alveolar and bronchiolar fate of BASC-derived cells.

BASCs contribute substantially to distal lung regeneration and display a bi-potent differentiation potential. Top: β‐galactosidase staining of cleared whole lung preparations isolated from control and injured BASC-specific animals (SPC^{-2A YFP-2A tTA N}, CCSP^{-2A mCherry-2A tTA C}, tetO_bi^luc/Cre, Rosa26^{stopflox-lacZ}) 21 days (bleomycin, naphthalene) or 35 days (influenza) following injury. Bottom: Sequential imaging of epifluorescence and β‐galactosidase activity, illustrating the alveolar and bronchiolar fate of BASC-derived cells.